MODULE 1: Two weeks (Without stem cell culture technique)
Batch 1: May 14th, 2012 onwards
Batch 2: June 4th, 2012 onwards
Fees: Rs. 7, 000
· Basic theory on stem cells
- Introduction
- Source and types of stem cells
- Embryonic versus adult stem cells
- Stem cells and Regenerative Medicine
· Isolation techniques
- Isolation of Mononuclear cells (MNC) from bone marrow and cord blood
- Isolation of Stromal vascular fraction (SVF) from adipose tissue
· Cell enumeration
- Cell counting using haemocytometer
- Cell viability test
· Flouresence activated cell sorting :BD-FACS ARIA
-Basic theory on flowcytometry
- Instrument demonstration
-Characterization of cell surface markers specific to stem cells
MODULE 2: Two weeks (With stem cell culturing technique)
Batch 1: May 14th, 2012 onwards
Batch 2: June 4th, 2012 onwards
Fees: Rs. 10, 000
· Basic theory on stem cells
- Introduction
- Source and types of stem cells
- Properties of stem cells
- Stem cells and Regenerative Medicine
-Insight on cell culture techniques
-Cancer stem cell
· Isolation techniques
- Isolation of Mononuclear cells (MNC) from bone marrow
- Isolation of Stromal vascular fraction (SVF) from adipose tissue
- Isolation of stem cells from novel sources
· Cell enumeration
- Cell counting using haemocytometer
- Cell viability test
· Flouresence activated cell sorting:BD-FACS ARIA
-Basic theory on flow cytometry
- Instrument demonstration
-Characterization of cell surface markers specific to stem cells
· Stem cell Culture Techniques
- Basic cell culture technique
- Formulation of different culture media
- Cell seeding and media change
- Passaging/ trypsinization of cells
- Freezing and thawing of cells
- Calculation of split ratio
- Overview on differentiation technique
MODULE 3: 1 WEEK ( Hands On Training on Flowcytometry – BD FACS Aria Cell sorter)
Hands on training will be given by Dr. M. Dhanasekaran, an expert in the field of cell culture and flowcytometry.
Duration: (May-June)
Fees: Rs. 10, 000
· Basic theory on stem cells
- Introduction
- Source and types of stem cells
- Properties of stem cells
- Stem cells and Regenerative Medicine
- Introduction to flowcytometry
- Overview on cell sorting
· Practicals
· Fluorescence activated cell sorting:BD-FACS ARIA cell Sorter
-Basic theory on flow cytometry
- Instrument demonstration
- Quality control
- Compensation
-Gating Strategies
- Cell viability testing
- Differential count analysis
- Immunophenotypic characterization of HSC markers
- Immunophenotypic characterization of MSC and endothelial population
ADVANCED POST GRADUATE DIPLOMA IN STEM CELL TECHNOLOGY AND RESEARCH (JOB ORIENTED)
SYLLABUS
This course was started with an envisage of training students in the field of stem cell technology with a special emphasis on imparting expertise hands on skills and extensive knowledge to enhance their career opportunities.
Duration: 1 Year Fee: Rs. 40, 000 per Semester
SYLLABUS
SEMESTER I:
Paper 1: Advanced cell and molecular Biology
Paper II: Molecular Developmental Biology
Paper III: Embryonic stem cell technology
Paper IV: Biostatisticsand Research Methodology
Paper V: Molecular Biology – Practicals
SEMESTER II:
Paper VI: Adult stem cell technology
Paper VII: Stem cells and Tissue Engineering
Paper VIII: Stem cell techniques (Practical)
Paper IX: Dissertation work
4. FINAL SEMESTER DISSERTATION/ MINI PROJECTS
DURATION: 1- 6 MONTHS FEE: 10, 000- 40, 000 per student
As final semester projects plays a very crucial time in student's caree, LIRM offers the best projects in stem cell technology field for the aspiring students. We take responsibility to train every student technical, research and thesis writing skill at the end of their projects.
TENTATIVE TOPICS AVAILABLE FOR PROJECTS:
1. Bone marrow derived stem cells - Isolation, culturing and characterization
2. Adipose tissue derived stem cells- Isolation, culturing and characterization
3. Role of Integrins
4. Role of Cell Adhesion Molecule (CAM) with ECM
5. Role of CAM in Wharton's jelly derived Mesenchymal stem cells
6. Research on Cancer stem cell markers
7. Sorting of MNC from Bone amrrow and cord blood using FACS
8. Sorting of MNC using MACS
9. Cord blood and whartons jelly derived Mesenchymal stem cells
10. Differentiation of BMSC and ADSC
11. Comparison of characterization of Side population and Endothelial Progenitor population using FACS
12. Culturing of Whartons jelly derived stem cells - Explant versus Enzymatic method.
13. Novel Sources of stem cells (isolation, characterization, culturing and differentiation of novel non-conventional ources of stem cells)
